HPLC (High-Performance Liquid Chromatography) is a technique used to separate, identify, and quantify compounds in a liquid sample. The separation is achieved by passing the sample through a column packed with a stationary phase. The different compounds in the sample will interact with the stationary phase to varying degrees, causing them to elute (come out of the column) at different times.
The choice of HPLC column is critical to the success of the separation. The column must be able to resolve the compounds of interest, and it must be compatible with the sample and the mobile phase (the liquid that carries the sample through the column).
